Stages

Specimens obtained from cavities or mucosae (sputum, stool, urine, mucus, pus, vaginal smear…) are prepared, and then put in contact with various nutrient mediums in Petri dishes. On the nutrient medium, pathogens develop rapidly.
Several series of small disks saturated with different essential oils are place on the microbial colonies.
After a latency period at 37, 5°, the diameter of the inhibition halo surrounding the disks can be measured in millimetres. This clear area represents the destruction of the germs and thus measures the effectiveness of the tested Essential Oils.

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This method is identical to the technique used to measure the bactericidal activity of antibiotics.